Quantitative analysis of nonsteroidal anti-inflammatory drugs in dried blood spot from mountain ultra-trail runners. Contribution of pharmacokinetic models for the interpretation of the results
Mashal et al.
The finding, in our words
A quantitative LC-MS/HRMS assay using dried blood spots was validated for 16 nonsteroidal anti-inflammatory drugs, achieving accuracy and imprecision within 15 percent for quality controls and below 20 percent at the lower limit of quantification. When applied to mountain ultra-trail runners, ibuprofen was the most frequently detected drug, and pharmacokinetic modelling established detection windows accounting for altered clearance during prolonged intense exercise.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
The analysis indicates that dried blood spots and volumetric absorptive microsampling enable minimally invasive monitoring of prohibited substances, with volumetric absorptive microsampling offering improved quantitative reliability over traditional dried spots.
A validated two-step LC-MS/MS protocol can identify 91 prohibited doping agents from a single dried blood spot collected on either cellulose cards or volumetric absorptive microsampling devices. This demonstrates robust multi-analyte screening from capillary blood, supporting decentralised anti-doping programmes where venous sampling is impractical.
This review highlights that microsampling techniques such as dried blood spots and volumetric absorptive microsampling offer superior alternatives to traditional sampling for the mass spectrometry detection of prohibited substances in anti-doping analysis. It provides guidance on selecting appropriate analytical methods and sample pretreatment strategies to ensure sensitive detection and reliable storage conditions.
Maykar et al., Critical reviews in analytical chemistry (paywalled) · source ↗
The authors validated a mass spectrometry method that simultaneously detects fifteen anabolic steroid esters and approximately eighty-five other prohibited substances from a single dried blood spot. Using a Tasso-M20 device, they detected testosterone undecanoate in authentic samples after oral administration, meeting World Anti-Doping Agency requirements. This reduces sample volume and simplifies workflows for decentralised anti-doping screening.
Sakellariou et al., Drug testing and analysis (paywalled) · source ↗
This study compared four dried blood spot devices for glucocorticoid detection, finding that the Mitra VAMS device offered the best combination of analytical recovery and user usability, whilst the HemaXis DB10 showed the least bias compared to whole blood.
Chen et al., Analytica chimica acta (paywalled) · source ↗