2023 · The Journal of molecular diagnostics : JMD · paywalled
Impact of Cell-Debris and Room-Temperature Storage on Urine Circulating Tumor DNA from Hepatocellular Carcinoma
Kim et al.
The finding, in our words
Cell debris can contain a substantial proportion of transrenal urine cell free DNA, and seven day room temperature storage does not degrade urine circulating tumour DNA quantity or size distribution compared with immediate freezing, supporting robust preanalytical handling for decentralised urine based liquid biopsy.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
The authors developed an integrated sequencing workflow that simultaneously profiles copy number aberrations and a three-gene methylation panel from home-collected urine cell-free DNA, achieving high diagnostic accuracy for both primary and recurrent bladder cancer in a cohort of 89 participants, which supports the feasibility of decentralised cancer detection from self-collected urine samples.
Beijert et al., JCO precision oncology (paywalled) · source ↗
Iron oxide nanoparticles with histidine and guanidine surfaces extracted urinary cell-free DNA at twice the yield of commercial kits, recovering more short fragments of 80 to 150 bp and achieving detection down to 10⁻³ ng/mL without specialised laboratory equipment. This approach could support at-home urine collection for non-invasive, longitudinal disease monitoring.
Haddad et al., Small methods (paywalled) · source ↗
This review summarises how emerging HPV detection methods including CRISPR/Cas systems, droplet digital PCR and isothermal amplification, combined with self-collected vaginal swabs and liquid biopsy from urine or blood, could improve screening acceptability and enable point-of-care testing compared with conventional approaches that require invasive sampling and centralised laboratory infrastructure.
Shi et al., Frontiers in cellular and infection microbiology · source ↗
This review surveys the use of tumour-derived cell-free DNA in urine for detecting non-urological cancers, covering methodological challenges such as DNA methylation analysis and transrenal transport mechanisms, and argues that urine self-collection could enable convenient home-based cancer screening at a curable stage.
Adding polyethylene glycol to first-void urine before centrifugation markedly improved cell-free DNA recovery in the pellet, whereas low-speed centrifugation alone removed cellular DNA while keeping cell-free DNA in the supernatant. Pseudovirions pelleted reliably under all conditions, but the study revealed substantial variation between self-collected samples, highlighting the need for standardised protocols in decentralised HPV screening programmes.
Téblick et al., European journal of medical research · source ↗