Dried urine spot (DUS) applied for sampling prior to the accurate HILIC-MS/MS determination of 14 amino acids
Meikopoulos et al.
The finding, in our words
A dried urine spot method for measuring 14 amino acids was validated using filter paper and mass spectrometry, offering a stable alternative to liquid urine that supports patient self-collection and remote analysis.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
Volumetric absorptive microsampling showed better precision than dried blood spots and a metabolic profile closer to whole blood, with stable signalling lipids for 24 hours at room temperature but significant changes after one week unless stabilised, indicating feasibility for decentralised sampling with the need for storage strategies.
Frequent dried blood spot sampling detected early changes in beta-cell function more effectively than mixed-meal tolerance tests or urine ratios, supporting its potential as a decentralised monitoring tool for clinical trials.
This review establishes that microsampling across blood, saliva, urine and stool matrices offers validated workflows and regulatory recognition for human biomonitoring comparable to conventional methods. It finds that these decentralised approaches enhance participant acceptability and enable screening in remote or low-resource settings.
The dried urine spot device showed strong agreement with liquid urine across three clinical chemistry analysers and stable performance across temperatures, enabling decentralised ambient collection for albumin-to-creatinine ratio.
Untargeted metabolomic profiles from three blood microsampling devices aligned more closely with whole blood than with plasma, and all devices distinguished sex based on amino acids, lipids, and acylcarnitines. This validates that device choice can be tailored to the metabolites of interest for decentralised human biomonitoring.