Development of objective indicators for quantitative analysis of sodium intake: the sodium to potassium ratio of second-void urine is correlated with 24-hour urinary sodium excretion

Adding polyethylene glycol to first-void urine before centrifugation markedly improved cell-free DNA recovery in the pellet, whereas low-speed centrifugation alone removed cellular DNA while keeping cell-free DNA in the supernatant. Pseudovirions pelleted reliably under all conditions, but the study revealed substantial variation between self-collected samples, highlighting the need for standardised protocols in decentralised HPV screening programmes.

Concentration strategies for spiked and naturally present biomarkers in non-invasively collected first-void urine — Téblick et al., European journal of medical research · source ↗

• first-void
• hpv
• liquid-biopsy
• liquid
• urine
• validation
• biomarkers

The review concludes that first-void urine is a feasible source for molecular biomarkers, such as host and viral gene methylation, to triage high-risk HPV infections, offering a non-invasive and preferred method for women that may improve screening adherence.

First-void urine: A potential biomarker source for triage of high-risk human papillomavirus infected women — Van Keer et al., European journal of obstetrics, gynecology, and reproductive biology (paywalled) · source ↗

• urine
• first-void
• self-collection
• validation
• hpv
• biomarkers
• liquid-biopsy

The study standardises leukocyte and interleukin-6 measurements in first-void urine, expressed prostatic secretion and post-massage urine from 541 adults and defines ROC-based cut-offs to distinguish inflammatory from non-inflammatory prostate disease, enabling more precise treatment selection in decentralised settings.

Inflammatory reaction found in prostate-specific material - method standardization and proposed optimal cut-off points — Korrovits et al., Andrology (paywalled) · source ↗

• first-void
• urine
• validation
• biomarkers

The authors developed and validated a chiral GC-MS method to quantify D-amino acid ratios in 1 mL first-void urine samples. In a study of 40 pregnant women, those with gestational diabetes mellitus showed altered urinary D-amino acid profiles compared to controls, suggesting that minimally invasive urine metabolomics could support decentralised screening for pregnancy-related metabolic disorders.

Optimization and validation of a chiral GC-MS method for the determination of free D-amino acids ratio in human urine: application to a gestational diabetes mellitus study — Lorenzo et al., Journal of pharmaceutical and biomedical analysis (paywalled) · source ↗

• urine
• first-void
• validation
• metabolome
• biomarkers

This meta-analysis found that HPV testing using self-collected vaginal and urine samples showed sensitivities of 88.6% and 81% for detecting cervical precancer. Using a standardised device for first-void urine increased sensitivity to 86.6%, supporting decentralised patient-centric screening, though data on specificity and specific commercial devices were not consistently reported.

Diagnostic Performance of HPV Testing Using Self-Collected Urine and Vaginal Samples for Detecting Cervical Precancer or Worse: A Meta-Analysis — Hsiao et al., The journal of obstetrics and gynaecology research (paywalled) · source ↗

• urine
• first-void
• self-collection
• validation
• hpv
• sti