Development and Validation of a Streamlined Workflow for Proteomic Analysis of Proteins and Post-translational Modifications from Dried Blood

Microsampling enables less invasive, patient-centric self-collection of capillary blood for remote monitoring of metabolites and lipids, overcoming conventional venipuncture constraints. Recent device innovations address dried blood spot limitations, particularly haematocrit and volume variations, expanding decentralised applications in population health, drug discovery and multi-omics research.

Revolutionizing Blood Collection: Innovations, Applications, and the Potential of Microsampling Technologies for Monitoring Metabolites and Lipids — Bossi et al., Metabolites · source ↗

• multimodal
• blood
• dbs
• metabolome
• self-collection
• haematocrit
• dried
• capillary
• validation
• biomarkers
• multi-omics

The analysis indicates that dried blood spots and volumetric absorptive microsampling enable minimally invasive monitoring of prohibited substances, with volumetric absorptive microsampling offering improved quantitative reliability over traditional dried spots.

Liquid Chromatographic Methods for Microsampling in Sports Drug Monitoring: Progress in DBS and VAMS — Zhang, Biomedical chromatography : BMC (paywalled) · source ↗

• vams
• doping
• blood
• dbs
• dried
• steroids
• toxicology
• hormones
• validation
• biomarkers

Researchers successfully validated an analytical method for measuring creatinine across plasma and volumetric absorptive microsampling devices, demonstrating strong correlation between conventional plasma and dried microsamples. This provides a reliable, patient-centric approach for monitoring kidney function remotely during transplant follow-up.

Surrogate-based LC-MS/MS quantification of endogenous creatinine in plasma, plasma-equivalent dried spots (qDPS), and volumetric absorptive microsampling (VAMS): an ICH M10-compliant bridging study — Kocur et al., European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (paywalled) · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• dried
• validation
• biomarkers

Volumetric absorptive microsampling showed better precision than dried blood spots and a metabolic profile closer to whole blood, with stable signalling lipids for 24 hours at room temperature but significant changes after one week unless stabilised, indicating feasibility for decentralised sampling with the need for storage strategies.

Volumetric absorptive microsampling for profiling of signaling lipids: a comparative analysis with whole blood and dried blood spots — Thangavelu et al., Analytical and bioanalytical chemistry · source ↗

• vams
• volumetric
• blood
• dbs
• metabolome
• dried
• validation
• biomarkers

An optimised VAMS workflow for blood proteomics increased protein identifications 4-fold in plasma and 2.1-fold in whole blood compared with liquid processing, with mean CVs below 11%, and whole blood showed greater robustness and storage stability at room temperature for up to 14 days. This enables reliable patient-centric microsampling for longitudinal biomarker studies.

Advancing Blood Proteome Analysis Past the Plasma Age: Mass Spectrometry of Whole Blood and Plasma Collected with Volumetric Absorptive Microsampling Devices — Karsten et al., Journal of proteome research (paywalled) · source ↗

• vams
• neoteryx-mitra
• blood
• dried
• validation
• biomarkers