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OpenSampling

2024 · BMC infectious diseases · open access

Clinic-based evaluation of the dual Xpert CT/NG assay on the GeneXpert System for screening for extragenital chlamydial and gonococcal infections amongst men who have sex with men

Cordioli et al.

The finding, in our words

The Xpert CT/NG assay showed high specificity and moderate-to-high sensitivity for chlamydia and gonorrhoea in urine and rectal specimens, though pharyngeal sensitivity was lower. High acceptability among men who have sex with men and rapid turnaround support its use for decentralised STI screening with immediate treatment initiation.

A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.

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  1. 2025

    Urine-based HPV testing demonstrated 74% sensitivity and 52% specificity for detecting CIN2+, with performance improving when first-void collection and PCR-based assays were used. Although less sensitive than clinician-collected cervical samples, urine testing was preferred by over 90% of women, indicating potential as a complementary screening strategy to improve uptake.

    Diagnostic Accuracy of Urine-Based HPV Testing Compared With Clinician-Collected Cervical Samples for Cervical Intraepithelial Neoplasia Grade 2 or Worse (CIN2+) Detection: A Systematic Review and Meta-AnalysisG et al., Cureus · source ↗

    • urine
    • first-void
    • self-collection
    • validation
    • acceptability
    • hpv
    • sti
  2. 2020

    First-void urine self-collection for HPV testing showed good agreement with cervical samples using the COBAS assay (kappa 0.66) but only moderate agreement with CLART (kappa 0.47). The COBAS assay achieved 63.9% sensitivity and 96.5% specificity with far fewer invalid results than CLART (1.8% versus 26.9%), and 42% of women preferred urine sampling, indicating that urine-based HPV testing is an acceptable decentralised option but requires improved analytical performance before implementation for non-participants.

    Urine collection in cervical cancer screening - analytical comparison of two HPV DNA assaysTranberg et al., BMC infectious diseases · source ↗

    • urine
    • first-void
    • self-collection
    • validation
    • acceptability
    • hpv
    • sti
  3. 2026

    This meta-analysis found that HPV testing using self-collected vaginal and urine samples showed sensitivities of 88.6% and 81% for detecting cervical precancer. Using a standardised device for first-void urine increased sensitivity to 86.6%, supporting decentralised patient-centric screening, though data on specificity and specific commercial devices were not consistently reported.

    Diagnostic Performance of HPV Testing Using Self-Collected Urine and Vaginal Samples for Detecting Cervical Precancer or Worse: A Meta-AnalysisHsiao et al., The journal of obstetrics and gynaecology research (paywalled) · source ↗

    • urine
    • first-void
    • self-collection
    • validation
    • hpv
    • sti
  4. 2026

    A study of 2500 women found 99.0% agreement between first-void urine and vaginal self-sampling for HPV detection using Cobas4800, with 98.1% of participants preferring urine. Although conducted in a resource-limited setting which may limit generalisability, these results support urine as a viable sample type for decentralised, patient-centric screening.

    Comparative efficacy, feasibility and acceptability of HPV DNA testing on first-void urine versus self-collected vaginal samples: a real-world study in a resource-limited settingBose et al., BMJ global health · source ↗

    • urine
    • first-void
    • self-collection
    • acceptability
    • hpv
    • sti
  5. 2026

    First-void urine and self-collected vaginal swabs showed similar sensitivity and specificity to clinician-collected cervical samples for high-risk HPV detection across two real-time PCR assays, supporting their use as decentralised, patient-collected alternatives; most women preferred first-void urine for regular cervical screening.

    Comparative performance of first-void urine and self-collected vaginal swabs against clinician-collected cervical samples using 2 HPV real-time assays in Indian womenA et al., Journal of gynecologic oncology (paywalled) · source ↗

    • acceptability
    • first-void
    • sti
    • self-collection
    • hpv
    • urine