A metabolomics approach to the identification of biomarkers of sugar-sweetened beverage intake

In adults given EPA or DHA for 12 weeks, first-void urine analysed by MSI-CE-MS showed increased S-carboxypropylcysteamine and other candidate metabolites that correlated with the Omega-3 Index measured in erythrocytes, suggesting urine could support non-invasive monitoring of omega-3 status.

Urinary Metabolite Profiling to Non-Invasively Monitor the Omega-3 Index: An Exploratory Secondary Analysis of a Randomized Clinical Trial in Young Adults — MacIntyre et al., Metabolites · source ↗

• urine
• first-void
• metabolome
• biomarkers

The authors developed and validated a chiral GC-MS method to quantify D-amino acid ratios in 1 mL first-void urine samples. In a study of 40 pregnant women, those with gestational diabetes mellitus showed altered urinary D-amino acid profiles compared to controls, suggesting that minimally invasive urine metabolomics could support decentralised screening for pregnancy-related metabolic disorders.

Optimization and validation of a chiral GC-MS method for the determination of free D-amino acids ratio in human urine: application to a gestational diabetes mellitus study — Lorenzo et al., Journal of pharmaceutical and biomedical analysis (paywalled) · source ↗

• urine
• first-void
• validation
• metabolome
• biomarkers

DNA methylation markers in first-void urine effectively detect high-grade cervical lesions in HPV-positive women, with results similar to clinician-collected samples in those over 30. This enables reliable cervical cancer screening using self-collected urine, reducing the need for invasive follow-up procedures in decentralised settings.

Evaluating DNA methylation markers and extended HPV genotyping in first-void urine for detecting high-grade cervical lesions in HPV-positive women: a cross-sectional study — Tranberg et al., BMC medicine · source ↗

• colli-pee
• first-void
• self-collection
• hpv
• urine
• biomarkers
• genotyping

The study found that testing for ASCL1/LHX8 DNA methylation in at-home collected first-void urine can detect most cervical cancers and high-grade precancers, supporting a fully molecular screening pathway without a clinical visit.

Clinical performance of ASCL1/LHX8 DNA methylation on first-void urine to screen for cervical cancer — Van Keer et al., Communications medicine · source ↗

• colli-pee
• first-void
• self-collection
• hpv
• liquid-biopsy
• urine
• biomarkers

A dried urine spot method for measuring 14 amino acids was validated using filter paper and mass spectrometry, offering a stable alternative to liquid urine that supports patient self-collection and remote analysis.

Dried urine spot (DUS) applied for sampling prior to the accurate HILIC-MS/MS determination of 14 amino acids — Meikopoulos et al., Talanta (paywalled) · source ↗

• dct
• metabolome
• dried
• urine
• validation
• biomarkers