A Book-Type Dried Plasma Spot Card for Automated Flow-Through Elution Coupled with Online SPE-LC-MS/MS Bioanalysis of Opioids and Stimulants in blood

This study compared four dried blood spot devices for glucocorticoid detection, finding that the Mitra VAMS device offered the best combination of analytical recovery and user usability, whilst the HemaXis DB10 showed the least bias compared to whole blood.

Analytical and usability comparison of microsampling dried blood spot devices for glucocorticoid detection in sports using ultra-high-performance liquid chromatography-tandem mass spectrometry — Chen et al., Analytica chimica acta (paywalled) · source ↗

• vams
• acceptability
• neoteryx-mitra
• doping
• blood
• dbs
• haematocrit
• dried
• toxicology
• hormones
• validation

The hemaPEN device demonstrated linear measurement of cocaine and metabolites in capillary blood with acceptable precision and accuracy, and analytes remained stable for seven days within the device. While haematocrit affected accuracy, it stayed within acceptable limits, suggesting the device could improve decentralised toxicology screening compared with conventional dried blood spot collection.

Evaluation of hemaPEN^® sampling device for measurement of cocaine and metabolites in capillary blood by LC-MS/MS — Smidt et al., Bioanalysis (paywalled) · source ↗

• blood
• dried
• capillary
• validation
• haematocrit
• toxicology

A VAMS method for 24 mycotoxins was validated against FDA and European Commission guidelines, showing no haematocrit bias and acceptable stability for 21 days at room temperature. Comparison with liquid whole blood from 20 samples revealed no missed exposed cases and comparable levels of key mycotoxins, supporting VAMS as an alternative to venous sampling in resource-limited settings.

Volumetric Absorptive Microsampling as an Alternative Tool for Biomonitoring of Multi-Mycotoxin Exposure in Resource-Limited Areas — Vidal et al., Toxins · source ↗

• blood
• dried
• vams
• validation
• venous-agreement
• haematocrit
• toxicology

The study developed and validated an LC-MS/MS method for THC, its metabolites and synthetic cannabinoids in both dried blood spots and VAMS, showing that VAMS eliminates the haematocrit effect that limits DBS accuracy, thereby providing a reliable tool for toxicological and anti-doping analysis in decentralised settings.

Dried haematic microsamples and LC-MS/MS for the analysis of natural and synthetic cannabinoids — Protti et al., Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (paywalled) · source ↗

• vams
• neoteryx-mitra
• doping
• blood
• dbs
• haematocrit
• dried
• toxicology
• validation

VAMS eliminated the variable haematocrit bias seen with DBS, but a residual haematocrit-dependent recovery effect persisted at high haematocrit: the nuance that assays still need per-analyte validation.

Does volumetric absorptive microsampling eliminate the hematocrit bias for caffeine and paraxanthine in dried blood samples? A comparative study — De Kesel, Lambert & Stove, Analytica Chimica Acta (paywalled) · source ↗

• vams
• blood
• dbs
• haematocrit
• dried
• toxicology
• validation