2021 · Journal of pharmaceutical and biomedical analysis · paywalled
VAMS and StAGE as innovative tools for the enantioselective determination of clenbuterol in urine by LC-MS/MS
Protti et al.
The finding, in our words
Protti and colleagues developed and validated a VAMS-based workflow for enantioselective analysis of clenbuterol in urine microsamples. The method achieved good linearity, a limit of quantification of 0.3 ng/mL, and 87% extraction yield, supporting its potential for decentralised doping control and toxicology screening.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
This review highlights that microsampling techniques such as dried blood spots and volumetric absorptive microsampling offer superior alternatives to traditional sampling for the mass spectrometry detection of prohibited substances in anti-doping analysis. It provides guidance on selecting appropriate analytical methods and sample pretreatment strategies to ensure sensitive detection and reliable storage conditions.
Dried urine microsampling with volumetric absorptive microsampling and dried urine spot improved the stability of peptide hormones and growth factors during drying, storage and transport, enabling reliable LC-MS/MS quantitation for anti-doping testing.
A direct DUS-versus-VAMS comparison for 13 steroids on the same urine: VAMS gave better precision and recovery and superior one-year room-temperature stability.
Dried urine spot and volumetric absorptive microsampling methods were validated for quantifying six corticosteroids in 30 μl urine, showing good linearity, extraction yields exceeding 81 percent, and precision within 15 percent relative standard deviation. Analytes remained stable for seven days at room temperature, indicating urine microsampling is viable for remote antidoping control and could support decentralised steroid monitoring.
The study developed and validated an LC-MS/MS method using volumetric absorptive microsampling to quantify cathinone analogues in dried urine, plasma and oral fluid, showing satisfactory linearity, precision, recovery, matrix effects, selectivity and stability, and was applied to real samples from users, offering a reliable decentralised sampling strategy for forensic toxicology and sport drug testing.