2014 · Climacteric : the journal of the International Menopause Society · paywalled
Lack of association between ESR1 gene polymorphisms and premature ovarian failure in Serbian women
Li et al.
The finding, in our words
This study genotyped two oestrogen receptor gene variants in DNA from saliva samples of Serbian women and found no association with premature ovarian failure. The use of Oragene kits for saliva collection shows a practical method for obtaining DNA in genetic research on reproductive conditions, though these specific markers appear uninformative in this population.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
Using the Oragene saliva device, this study identified elevated CYP2C8 allele frequencies in a Saudi paediatric cohort compared to other Asian populations, suggesting potential variability in ibuprofen metabolism and drug response.
Using the Oragene Discovery 500 series for decentralised saliva collection in 524 African American adolescents, this study found that positive neighbourhood conditions relate to fewer behavioural difficulties, despite genetic risk. The authors note that most gene-environment studies of behaviour use European ancestry samples, limiting generalisability to African American youth.
Using patient-centric saliva microsampling with the Oragene kit, the study found a low overall prevalence, 13%, of the OPRM1 118G allele in a Western Saudi population, with a significantly higher frequency in females, 21%, than males, 5%. The authors note the findings are specific to this population and may not be generalisable.
The study found that the Oragene OG-600 receptacle provided the highest concentration of salivary DNA, including short fragments suitable for analysis, and successfully detected HPV DNA from patient saliva. This matters for decentralised sampling as it supports the use of a standardised, self-collection device for non-invasive cancer biomarker detection.
Self-collected saliva samples provided adequate DNA for genotyping in preterm birth research, though questionnaire data on birth details had limited concordance with prospective records, indicating that while DNA sampling is feasible via post, phenotyping requires more reliable data collection methods for genetic studies.