Dried blood spot analysis with liquid chromatography and mass spectrometry: Trends in clinical chemistry
Skogvold et al.
The finding, in our words
This review confirms that dried blood spot sampling with mass spectrometry enables simple preparation and transport of blood for clinical analysis, supporting decentralised diagnostics from newborn screening to therapeutic drug monitoring. Emerging untargeted metabolomics and proteomics applications, plus novel on-paper preparation methods, promise to expand microsampling utility in remote and resource-limited settings.
A paraphrase to the Library’s standard, never the abstract. The source is one link away and is always the authority.
In AML and CLL, 91% of VAMS venetoclax results fell within 20% of plasma after individualised haematocrit correction; in home sampling, 18 of 21 patients self-sampled independently and 76% of returned samples were analysable, which shows home microsampling is workable, though the authors ask for multicentre validation.
Levens et al., Clinical Pharmacokinetics (paywalled) · source ↗
The analysis indicates that dried blood spots and volumetric absorptive microsampling enable minimally invasive monitoring of prohibited substances, with volumetric absorptive microsampling offering improved quantitative reliability over traditional dried spots.
This review found that volumetric microsampling devices, such as Mitra, HemaPEN, HemaXis DB10, and Tasso-M20, overcome haematocrit bias, whilst Telimmune plasma separation cards enable direct plasma collection without centrifugation. These technologies support patient-centric, decentralised sampling, though the authors note that clinical validation remains limited across different drugs and patient populations.
Huhn & Scherf-Clavel, Therapeutic drug monitoring (paywalled) · source ↗
Volumetric absorptive microsampling showed better precision than dried blood spots and a metabolic profile closer to whole blood, with stable signalling lipids for 24 hours at room temperature but significant changes after one week unless stabilised, indicating feasibility for decentralised sampling with the need for storage strategies.
Thangavelu et al., Analytical and bioanalytical chemistry · source ↗
Capillary dried blood spots, after haematocrit-dependent conversion, showed good agreement with plasma for 25-hydroxyvitamin D quantification, with 90 per cent of results within 20 per cent of plasma and substantial to almost perfect agreement in status classification, supporting reliable home self-collection for large-scale vitamin D monitoring.
Heughebaert et al., Clinical chemistry and laboratory medicine (paywalled) · source ↗