A targeted UHPLC-MS/MS method to monitor lipidomic changes during a physical effort: Optimization and application to blood microsamples from athletes

Plasma lipidomes from self-collected upper-arm capillary blood were statistically indistinguishable from paired venous plasma, most classes at r=0.95–0.99, validating at-home capillary self-collection for lipidomics.

Comparison of capillary and venous plasma lipidomes: validation of self-collected blood for plasma lipidomics — Hameed et al., J. Lipid Research · source ↗

• venous-agreement
• blood
• metabolome
• self-collection
• capillary
• liquid
• validation
• biomarkers

Capillary blood collection using volumetric absorptive microsampling demonstrated equivalent quantification of principal colonic polyphenol metabolites compared with matched venous plasma across a six-hour period following barley biscuit consumption. The technique successfully captured the pharmacokinetic profiles over 48 hours, confirming VAMS as a reliable alternative to venepuncture for dietary biomarker monitoring.

Finger-prick blood sampling using volumetric absorptive microsampling (VAMS) method for monitoring the main (poly)phenolic metabolites in human blood after barley biscuit intake — Cortijo-Alfonso et al., Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (paywalled) · source ↗

• vams
• venous-agreement
• neoteryx-mitra
• blood
• metabolome
• dried
• capillary
• validation
• biomarkers

Microsampling enables less invasive, patient-centric self-collection of capillary blood for remote monitoring of metabolites and lipids, overcoming conventional venipuncture constraints. Recent device innovations address dried blood spot limitations, particularly haematocrit and volume variations, expanding decentralised applications in population health, drug discovery and multi-omics research.

Revolutionizing Blood Collection: Innovations, Applications, and the Potential of Microsampling Technologies for Monitoring Metabolites and Lipids — Bossi et al., Metabolites · source ↗

• multimodal
• blood
• dbs
• metabolome
• self-collection
• haematocrit
• dried
• capillary
• validation
• biomarkers
• multi-omics

Capillary VAMS blood covered more of the metabolome than plasma, capturing intracellular red-cell metabolites, and correlated moderately-to-well with venous, Spearman 0.66; the honest caveats are higher finger-prick replicate variability and a need for −80 °C storage within six hours.

VAMS-based blood capillary sampling for mass-spectrometry-based human metabolomics studies — Volani et al., Metabolites · source ↗

• vams
• venous-agreement
• blood
• metabolome
• dried
• capillary
• validation
• biomarkers

The authors validated a UHPLC-MS/MS method for quantifying four ceramide species in 10 μL quantitative dried blood spots. Ceramides remained stable at room temperature, supporting remote self-collection, and concentrations were higher in fingertip whole blood than in plasma or serum, which is relevant for at-home monitoring of cardiovascular and metabolic disease risk.

Ceramides biomarkers determination in quantitative dried blood spots by UHPLC-MS/MS — Meikopoulos et al., Analytica chimica acta (paywalled) · source ↗

• blood
• qdbs
• metabolome
• self-collection
• dried
• capillary
• validation
• biomarkers